← Reference library

Metabolic

AICAR

Also known as Acadesine, AICA riboside, AICA ribonucleotide, 5-Aminoimidazole-4-carboxamide riboside

An AMP-mimetic nucleotide that activates AMPK and acts as an 'exercise mimetic' in animals; it is banned by WADA and has little human performance data.

Molecular weight258.23 g/mol (C9H14N4O5; AICA riboside form)
Half-life~1–2 hours (short).
CategoryMetabolic

Typical use cases

✓ AMPK/metabolic research✓ Endurance/exercise-mimetic studies✓ Glucose-uptake and fat-oxidation research

Dosing guidelines

StartingResearch context: anecdotal protocols cite ~10–25 mg/day SC; no established human protocol.
Maintenance~25–50 mg/day in informal research use (not standardized or validated).
Max (studied)Undefined for chronic SC use; IV acadesine trials used gram-level infusions in hospital settings — not comparable to research SC dosing.

Titration

Typically flat-dosed; the short half-life sometimes prompts split daily dosing in informal protocols.

Reconstitution

50 mg vial: add ~2.5–5 mL bacteriostatic water (~10–20 mg/mL); swirl gently.

Injection sites

Subcutaneous, rotate sites (abdomen/thigh); clinical acadesine studies used IV.

Storage & handling

Lyophilized: stable months cool/dark, longer frozen. Reconstituted: refrigerate (2–8 °C).

Side effects

  • Gastrointestinal upset Mild · Uncommon
  • Transient hypoglycemia Moderate · Rare
  • Elevated uric acid / lactic acid Moderate · Uncommon
  • Fatigue / headache Mild · Uncommon

Research summary

AICAR (acadesine) is phosphorylated intracellularly to ZMP, an AMP analog that allosterically activates AMP-activated protein kinase (AMPK), driving mitochondrial biogenesis (PGC-1α), insulin-independent glucose uptake, and fatty-acid oxidation. A 2008 Cell paper (Narkar et al.) showed AICAR boosted running endurance in sedentary mice, popularizing the 'exercise mimetic' label; it has been studied clinically as IV acadesine, but evidence for the research-peptide endurance/fat-loss use is largely animal-based and thin in humans. It has been on the WADA prohibited list since around 2009. Educational only — not medical advice.

Key references

  • Narkar et al., Cell (2008) — AMPK and PPARδ agonists as exercise mimetics
  • Corton et al., Eur. J. Biochem. (1995) — AICAR as a cell-permeable AMPK activator

Related compounds

Where to buy

These are affiliate links. Purchasing through them helps support DoseForge at no extra cost to you.

Educational reference only — not medical advice. Dosing figures are commonly-cited ranges, not prescriptions. Consult a qualified clinician.

Track AICAR in DoseForge

Log doses, rotate sites, watch your progress — free.

Get the app